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AGWAS-EPNs: Application of Genome-Wide Analysis Study to discriminate Entomopathogenic Nematodes

Ref.º FCT- 2022.06153.PTDC
National research project
From 2023 to 2024

Summary

Entomopathogenic nematodes are biological control agents of great potential against different pests. However, the phenotypic and genetic diversity of these nematodes requires careful selection. Normally, this selection is made using laboratory tests, which require keeping the target insects in the laboratory for long periods and in large numbers, which makes the process difficult. Recent advances in the knowledge of these organisms’ genetics open the perspective of the use of molecular approaches in the characterization of phenotypes based on the determination of haplotypes. This is the case of single nucleotide polymorphisms (SNPs) which have already shown their potential in entomopathogenic nematodes. Essentially, the project will increase the number of isolates, test their virulence against a pest that we have chosen as a target (Popillia japonica, an emerging pest in mainland Europe and installed in some islands of the Azores since the last century). Then, genotype these isolates and correlate the virulence with genotype, in order to identify the molecular marker(s) of the virulent nematode and then validate the proposed marker(s).

The project will provide an applicated tool based on molecular techniques with the potential to reduce the experimental effort to detect virulent strains. Besides, the methodology of quick detection of EPNs strains for a defined trait can be replicated for different pests or to perform a screening of wild strains.

Funding Institution

FCT - Fundação para a Ciencia e Tecnologia

Partners

Fundação Gaspar Frutuoso

E-NEMA GmbH (Germany) (Collaboration institution)

Project Coordinator